rabbit anti papss2 proteintech  (Proteintech)

Journal: Frontiers in Genetics

Article Title: A metabolism-related gene signature for predicting the prognosis in thyroid carcinoma

doi: 10.3389/fgene.2022.972950

Figure Lengend Snippet: Identification and functional enrichment of differentially expressed genes. (A) A volcano map of differential expression genes showed 120 upregulated and 81 downregulated genes. Red represents upward regulation in tumor tissue, while blue represents downward regulation. PAPSS2, CYP1A1, and ITPKA were labeled in the figure to indicate their expression trends in tumor samples. In addition, genes with prognostic significance and | logFC | greater than 2 were specifically marked. (B) A heat map diagram of differential gene expression in tumors and normal samples. (C) The significant GO terms were depicted as a heat map with color annotation of up-regulated or down-regulated genes. (D) The results of KEGG enrichment analysis were demonstrated in the circle diagram, each trapezoid representing a KEGG pathway, where red and blue dots represented up-regulated and downregulated genes respectively.

Article Snippet: Cells were incubated overnight with primary antibodies at 4°C and secondary antibody (GB21303, 1:300, Servicebio, China) for 1 h. Here are the primary antibodies we used: CYP1A1 (PB9544, 1:200, Boster, China), ITPKA (14270-1-AP, 1:100, Proteintech, China), PAPSS2 (CSB-PA527262LA01HU, 1:100, Cusabio, China).

Techniques: Functional Assay, Quantitative Proteomics, Labeling, Expressing, Gene Expression

Journal: Frontiers in Genetics

Article Title: A metabolism-related gene signature for predicting the prognosis in thyroid carcinoma

doi: 10.3389/fgene.2022.972950

Figure Lengend Snippet: The expression levels of CYP1A1 (A) , ITPKA (B) and PAPSS2 (C) in tumor and normal cells. (D – F) Immunofluorescence staining of normal thyroid cells and PTC cells to further confirm differential expression.

Article Snippet: Cells were incubated overnight with primary antibodies at 4°C and secondary antibody (GB21303, 1:300, Servicebio, China) for 1 h. Here are the primary antibodies we used: CYP1A1 (PB9544, 1:200, Boster, China), ITPKA (14270-1-AP, 1:100, Proteintech, China), PAPSS2 (CSB-PA527262LA01HU, 1:100, Cusabio, China).

Techniques: Expressing, Immunofluorescence, Staining, Quantitative Proteomics

Journal: F1000Research

Article Title: Validation of commercially available sphingosine kinase 2 antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence

doi: 10.12688/f1000research.10336.2

Figure Lengend Snippet: Details of primary and secondary antibodies.

Article Snippet: Blots were probed with Proteintech rabbit polyclonal anti-SK2 antibody ( A–D ) or ECM Biosciences rabbit polyclonal anti-SK2 antibody ( E–H ).

Techniques:

Journal: F1000Research

Article Title: Validation of commercially available sphingosine kinase 2 antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence

doi: 10.12688/f1000research.10336.2

Figure Lengend Snippet: Immunoblot analyses of lysates from HEK293 and HeLa cells treated with scrambled control siRNA (si-Neg) or SK2 siRNA (si-SK2), and lysates from wildtype (WT) or Sphk2 -/- MEFs. An equal amount (40 µg) of total protein from each sample was run in duplicate. After transferring to nitrocellulose and blocking, the membrane was separated and duplicate samples were probed with either ( A ) Proteintech rabbit anti-SK2 antibody or ( B ) ECM Biosciences rabbit anti-SK2 antibody. SK2 membranes were imaged using a 4 min exposure. The expected band size for SK2 is ∼65 kDa. Membranes were re-probed with mouse anti-α-tubulin antibody as a loading control (2 min exposure), which was detected at 55 kDa as expected. Consistent results were observed from 2-3 (HEK293 and MEF) or 3-4 (HeLa) independent experiments for each antibody.

Article Snippet: Blots were probed with Proteintech rabbit polyclonal anti-SK2 antibody ( A–D ) or ECM Biosciences rabbit polyclonal anti-SK2 antibody ( E–H ).

Techniques: Western Blot, Control, Transferring, Blocking Assay, Membrane

Journal: F1000Research

Article Title: Validation of commercially available sphingosine kinase 2 antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence

doi: 10.12688/f1000research.10336.2

Figure Lengend Snippet: SK2 was immunoprecipitated from HEK293 cell lysate using either ( A ) Proteintech rabbit anti-SK2 antibody or ( B ) ECM Biosciences rabbit anti-SK2 antibody. Normal rabbit IgG antibody was used as an isotype control. Immunoprecipitates (and 40 µg lysate input) were subjected to immunoblot analyses and probed with ( A ) Proteintech rabbit anti-SK2 antibody or ( B ) ECM Biosciences rabbit anti-SK2 antibody. Membranes were imaged using a 4 min exposure. Images are representative of three independent experiments for each antibody. ( C ) SK2 was immunoprecipitated from HEK293 cell lysates (of equal protein) that had been treated with scrambled control siRNA (si-Neg) or SK2 siRNA (si-SK2), using ECM Biosciences rabbit anti-SK2 antibody. Immunoprecipitates were subjected to immunoblot analyses and probed with ECM Biosciences rabbit anti-SK2 antibody. Membrane was imaged using a 4 min exposure. Image is representative of three independent experiments. IgG h/c = IgG heavy chain.

Article Snippet: Blots were probed with Proteintech rabbit polyclonal anti-SK2 antibody ( A–D ) or ECM Biosciences rabbit polyclonal anti-SK2 antibody ( E–H ).

Techniques: Immunoprecipitation, Control, Western Blot, Membrane

Journal: F1000Research

Article Title: Validation of commercially available sphingosine kinase 2 antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence

doi: 10.12688/f1000research.10336.2

Figure Lengend Snippet: ( A ) HeLa or ( B ) HEK293 cells were treated with scrambled control siRNA (si-Neg) or SK2 siRNA (si-SK2), and endogenous SK2 (green) was visualised by immunofluorescence staining and confocal microscopy, using Proteintech rabbit anti-SK2 antibody or ECM Biosciences rabbit anti-SK2 antibody. ( C ) Wildtype (WT) or Sphk2 -/- MEFs were seeded, and endogenous SK2 (green) was visualised by immunofluorescence staining and confocal microscopy, using Proteintech rabbit anti-SK2 antibody or ECM Biosciences rabbit anti-SK2 antibody. Nuclei were stained with DAPI (blue). For each cell line, background staining was examined by staining cells (si-Neg or WT cells) with secondary antibody and DAPI only, and collecting images using both 488nm and 405nm lasers (SK2 + DAPI). Images were taken at 40× magnification; scale bars = 10 µm. Images shown are representative of more than 100 cells from each experiment, and these results were consistent over three independent experiments for each cell line.

Article Snippet: Blots were probed with Proteintech rabbit polyclonal anti-SK2 antibody ( A–D ) or ECM Biosciences rabbit polyclonal anti-SK2 antibody ( E–H ).

Techniques: Control, Immunofluorescence, Staining, Confocal Microscopy